Journal: Experimental and Therapeutic Medicine

Article Title: Inhibition of the NMDA receptor protects the rat sciatic nerve against ischemia/reperfusion injury

doi: 10.3892/etm.2016.3148

Figure Lengend Snippet: Effects of MK-801 on tumor necrosis factor (TNF)-α protein expression levels in the rat sciatic nerve (SN) following ischemia/reperfusion (I/R) injury. Protein expression levels of TNF-α were determined using immunohistochemistry (magnification, ×400). (A) No TNF-α expression was detected in the Schwann cells derived from the SN of a sham-operated rat. (B) Moderate protein expression levels of TNF-α were detected in the Schwann cells derived from the SN fiber of a rat in the 12 h post-reperfusion I/R subgroup. (C) Higher protein expression levels of TNF-α were detected in the Schwann cells derived from the SN of a rat in the 24 h post-reperfusion I/R subgroup. (D) Numerous inflammatory cells had infiltrated the area surrounding the Schwann cells and moderate protein expression levels of TNF-α were detected in the SN of a rat in the 72 h post-reperfusion I/R subgroup. (E) Widespread demyelination and mild-to-moderate TNF-α protein expression levels in Schwann cells were detected in the SN derived from a rat in the 7 days post-reperfusion I/R subgroup. (F) A SN from a rat in the I/R + MK-801 group at 12 h post-reperfusion exhibited mild-to-moderate TNF-α protein expression levels in Schwann cells. (G) A SN from a rat in the I/R + MK-801 group at 24 h post-reperfusion exhibited markedly fewer infiltrating cells, as compared with the SN derived from I/R rats at the same time point post-reperfusion. Moderate protein expression levels of TNF-α expression were observed. (H) A SN derived from a rat in the I/R + MK-801 group at 7 days post-reperfusion. As compared with the SNs derived from the I/R rats, the extent of demyelination was markedly reduced and Schwann cells exhibited only low protein expression levels of TNF-α.

Article Snippet: Tissue slices were incubated with rabbit anti-mouse TNF-α polyclonal antibody (1:100; BA14901; Wuhan Boster Bio-Engineering Co., Ltd.) at 4°C overnight.

Techniques: Expressing, Immunohistochemistry, Derivative Assay

Journal: Experimental and Therapeutic Medicine

Article Title: Inhibition of the NMDA receptor protects the rat sciatic nerve against ischemia/reperfusion injury

doi: 10.3892/etm.2016.3148

Figure Lengend Snippet: Protein expression levels of tumor necrosis factor-α in the various treatment subgroups were quantified using the integrated optical density method, and are presented as the mean ± standard deviation (n=6). Δ P<0.05, ΔΔ P<0.01 vs. the I/R group. I/R, ischemia reperfusion.

Article Snippet: Tissue slices were incubated with rabbit anti-mouse TNF-α polyclonal antibody (1:100; BA14901; Wuhan Boster Bio-Engineering Co., Ltd.) at 4°C overnight.

Techniques: Expressing, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Inhibition of the NMDA receptor protects the rat sciatic nerve against ischemia/reperfusion injury

doi: 10.3892/etm.2016.3148

Figure Lengend Snippet: Effects of MK-801 on TNF-α and TACE mRNA expression levels in the rat sciatic nerve (SN) following ischemia/reperfusion (I/R) injury. TNF-α and TACE mRNA expression levels were determined using reverse transcription-quantitative polymerase chain reaction and are expressed relative to β-actin. Agarose gel images showing TNF-α and TACE mRNA expression levels in the SN homogenates at (A) 0, (B) 6, (C) 12, (D) 24 and (E) 72 h and (F) 7 days post-reperfusion. β-actin=280 bp; TNF-α=402 bp; TACE=624 bp. Relative (G) TNF-α and (H) TACE mRNA expression levels are presented as the mean ± standard deviation (n=6). *P<0.05, **P<0.01 vs. the sham-operated group; Δ P<0.05, ΔΔ P<0.01 vs. the I/R group. TNF-α, tumor necrosis factor-α; TACE, TNF-α-converting enzyme.

Article Snippet: Tissue slices were incubated with rabbit anti-mouse TNF-α polyclonal antibody (1:100; BA14901; Wuhan Boster Bio-Engineering Co., Ltd.) at 4°C overnight.

Techniques: Expressing, Reverse Transcription, Real-time Polymerase Chain Reaction, Agarose Gel Electrophoresis, Standard Deviation

Journal: Molecules

Article Title: Astragalus membranaceus Extract Activates Immune Response in Macrophages via Heparanase

doi: 10.3390/molecules17067232

Figure Lengend Snippet: Effects of anti-HPA antibody on the expression of IL-1β and TNF-α in AME-treated macrophages. Cells were cultured with AME, pre-treated with anti-HPA antibody for 1 h before exposed to AME for 24 h. The secretion of IL-1β and TNF-α were measured by using ELISA ( A ); The mRNA levels of IL-1β and TNF-α were measured by RT-PCR ( B ). * p < 0.05 compared to control and # p < 0.05 compared to AME.

Article Snippet: Heparan degrading enzyme assay kit was from Takara Bio Inc. Antibody against HPA, ELISA kits for mouse TNF-α and IL-1β were from Wuhan Boster Bio-engineering Limited Company (Wuhan, China).

Techniques: Expressing, Cell Culture, Enzyme-linked Immunosorbent Assay, Reverse Transcription Polymerase Chain Reaction, Control

Journal: International Journal of Nanomedicine

Article Title: Breast Cancer Chemoprevention from Nano Zingiber officinale Roscoe

doi: 10.2147/IJN.S474611

Figure Lengend Snippet: SOD, GSH, CAT, and TNF-α Levels in Blood Serum of Female Mus Musculus Balb/c

Article Snippet: The substances used in the experiment include κ-carrageenan, distilled water, methanol, benzo[α]pyrene (Merck), ethanol, Neutral Buffered Formalin (NBF) 10%, buffer solutions with pH values of pH 2 (HCl (Merck) – KCl (Merck)), 7 (Phosphate buffer (Merck)), and 8.5 (H 3 BO 3 (Merck) – NaOH (Merck)), phosphate buffer saline (PBS) (Merck), CAT kit (BT-Lab E0075Mo), GSH kit (BT-Lab EA0104Mo), SOD kit (BT-Lab E0290Mo), TNF-α kit (BT-Lab E0117Mo), and Na-CMC (Sigma).

Techniques:

Journal: International journal of molecular sciences

Article Title: Preventive Effects of Rhodiola rosea L. on Bleomycin-Induced Pulmonary Fibrosis in Rats.

doi: 10.3390/ijms17060879

Figure Lengend Snippet: Figure 5. Effects of RRL on the levels of α-smooth muscle actin (α-SMA) in the lung tissues after bleomycin (BLM)-induced in rats. (a) representative immunohistochemistry image (Bar = 50 µm). (A) normal group; (B) model group; (C) PAG group; (D–F) are RRL groups treated with 125, 250 and 500 mg/kg, respectively; (G) negative control of omitted first antibody; (H) negative control of omitted second antibody; (b) the quantitative analysis of α-SMA protein in lung tissues. Data represent the mean ˘ standard deviation (SD) (n = 3) (** p < 0.01 vs. normal group, # p < 0.05, ## p < 0.01, vs. model group).

Article Snippet: Mouse anti-α-SMA monoclonal antibody (Boster Biological Technology, Pleasanton, CA, USA), rabbit anti-MMP-9 polyclonal antibody, rabbit anti-TGF-β1 polyclonal antibody (Boster Biological Technology) and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG antibody (Boster Biological Technology) were purchased and used as received.

Techniques: Immunohistochemistry, Negative Control, Standard Deviation

Journal: International journal of molecular sciences

Article Title: Preventive Effects of Rhodiola rosea L. on Bleomycin-Induced Pulmonary Fibrosis in Rats.

doi: 10.3390/ijms17060879

Figure Lengend Snippet: Figure 8. Expression level of TIMP-1, MMP-9 and α-SMA of the lung tissues in rats. (A) normal group; (B) model group; (C) PAG group; (D–F) are RRL groups treated with 125, 250 and 500 mg/kg, respectively.

Article Snippet: Mouse anti-α-SMA monoclonal antibody (Boster Biological Technology, Pleasanton, CA, USA), rabbit anti-MMP-9 polyclonal antibody, rabbit anti-TGF-β1 polyclonal antibody (Boster Biological Technology) and horseradish peroxidase (HRP)-labeled goat anti-rabbit IgG antibody (Boster Biological Technology) were purchased and used as received.

Techniques: Expressing